Pectolyase Y-23, A. japonicus is an enzyme derived from Aspergillus japonicas. Pectolyase Y-23, A. japonicus can be used in combination with cellulase to isolate bioactive protoplasts[1].
References
[1] Yang D, et al. A high-efficiency PEG-Ca2+-mediated transient transformation system for broccoli protoplasts. Front Plant Sci. 2022 Dec 12;13:1081321. DOI:10.3389/fpls.2022.1081321