ChemicalBook CAS DataBase List 9025-65-4

9025-65-4

Name	MUNG BEAN NUCLEASE
CAS	9025-65-4
EINECS(EC#)	253-443-9
MDL Number	MFCD00131010

Chemical Properties

storage temp.	-20°C
form	lyophilized powder
color	Colorless to light yellow
PH	8.0 (25°C in water)
biological source	Serratia marcescens
Specific Activity	≥250units, pH 8.0, 37°C
Major Application	pharma/biopharma processes

Safety Data

Safety Statements	23-24/25 less more
WGK Germany	3
F	10-21
Storage Class	10 - Combustible liquids

Hazard Information

Uses

The enzyme from Sigma has been used to limit cell clumping during the preparation of chimeric cell mixtures. It has also been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA.

Uses

The enzyme has been used for an assay to detect the amount of Tf1 RNA that is protected from degradation by the nuclease, Benzonase. The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. It has also been used in the fractionation of nuclear pellet and nuclear extract obtained from cell lysates.

Uses

Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.

General Description

Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.

Biochem/physiol Actions

Benzonase? is a genetically engineered endonuclease from Serratia marcescens. The protein is a dimer of 30 kDa subunits with two essential disulfide bonds. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for the removal of nucleic acids from recombinant proteins. It can also be used for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping. Pre-treatment of a protein sample with this enzyme improves its resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. The optimum pH for enzyme activity is found to be 8.0-9.2.

Supplier

Jiangsu Jinpu Nuoan Biotechnology Co., Ltd

Telephone0512-82709698 15250590826

Websitehttps://www.gproan.com.cn/

Shanghai Surzyme Biotech Co.Ltd.

Telephone400 021 5689 18621959160

Websitehttps://www.surzyme.com

Shanghai ZymeTree Biotech Co.,Ltd.

Telephone 15300487802

Websitehttps://www.zymetree-bio.com

Nanjing Yaozheng Pharmaceutical Technology Co., Ltd.

Telephone 17721507860

Website

Shanghai Baitai Xin Biotechnology Co., Ltd.

Telephone 13701899122

Website

Nanjing Dulai Biotechnology Co., Ltd.

Telephone025-84699383-8003;025-846993838003-8003 18013301590

Websitehttp://www.njduly.com

Nanjing Sunlida Biological Technology Co., Ltd.

Telephone025-57798810 18652991625

Website

Shanghai Macklin Biochemical Co.,Ltd.

Telephone15221275939 15221275939

Websitehttp://www.macklin.cn

Shanghai Baoman Biotechnology Co., Ltd.

Telephone021-62130998

Websitehttp://www.baomanbio.com

Sigma-Aldrich

Telephone021-61415566 800-8193336

Websitehttps://www.sigmaaldrich.cn

Hubei kangen Pharmaceutical Chemical Co., Ltd

Telephone180-64105258 18064105258

Websitehttps://www.chemicalbook.com/supplier/10564672/

Shanghai YuanYe Biotechnology Co., Ltd.

Telephone 13636370518

Websitehttps://www.shyuanye.com

Chengdu Youngshe Chemical Co., Ltd.

Telephone+86-17380623303

Websitehttps://shop326109856.taobao.com/

Beijing OKA biological technology co., LTD

Telephone010-62971590 18548936886

Websitehttp://www.bjoka-vip.com/

Guangzhou Tomums Life Science Co., Ltd.

Telephone020-31155029 18902330969

Websitehttp://www.tomums.cn/

Hubei Weideli Chemical Reagent Co., Ltd.

Telephone13429867250 13429867250

Websitehttp://www.dxtyaoye.com

Wuhan Qingshan Heyuan Biotechnology Co., Ltd

Telephone027-18327024450 18327024450

Website

career henan chemical co

Telephone+86-0371-86658258

Websitehttp://www.coreychem.com

1of4

Send Inquriy