ChemicalBook CAS DataBase List 880107-52-8

880107-52-8

Name	Psalmotoxin 1
CAS	880107-52-8

Chemical Properties

form	Solid
color	White to off-white

Hazard Information

Uses

Psalmotoxin 1 (PcTx1) is a protein toxin that can bind at subunit-subunit interfaces of acid-sensing ion channel 1a (ASIC1a). Psalmotoxin 1 is a potent and slective ASIC1a inhibitor (IC50: 0.9 nM) by increasing the apparent affinity for H+ of ASIC1a. Psalmotoxin 1 can induce cell apoptosis, also inhibits cell migration, proferliration and invasion of cancer cells. Psalmotoxin 1 can be used in the research of cancers, or neurological disease[1][3][4][6].

in vivo

Psalmotoxin 1 (i.c.v., 1 ng/kg, a single dose) is neuroprotective in a conscious model of stroke via direct inhibition of ASIC1a^[2].
? Psalmotoxin 1 (tail vein injection, 10 ng/kg, daily for 7 days) inhibits tumor growth in breast cancer mice model^[4].

Animal Model:	Male spontaneously hypertensive rats (SHR)^[2]
Dosage:	1 ng/kg, a single dose.
Administration:	Intracerebroventricular (i.c.v.) injection
Result:	Reduced cortical and striatal infarct volumes measured 72 h post-stroke. Reduced the severity of motor deficit at 1 and 3 days after stroke compared to control rats. Displayed an anti-apoptotic effect in the occluded hemisphere (reduced stroke-induced caspase-3 positive cells).

Animal Model:	Female nude BALB/C mice (orthotopic implantation, MCF-7 and MDA-MB-231 cells)^[3]
Dosage:	10 ng/kg, daily for 7 days.
Administration:	Tail vein injection
Result:	Inhibited breast tumor growth.

References

[1] Chen X, et al. The tarantula toxin psalmotoxin 1 inhibits acid-sensing ion channel (ASIC) 1a by increasing its apparent H+ affinity. J Gen Physiol. 2005 Jul;126(1):71-9. DOI:10.1085/jgp.200509303
[2] Claudia A McCarthy, et al. PcTx1 affords neuroprotection in a conscious model of stroke in hypertensive rats via selective inhibition of ASIC1a. Neuropharmacology. 2015 Dec;99:650-7. DOI:10.1016/j.neuropharm.2015.08.040
[3] Niko Joeres, et al. Functional and pharmacological characterization of two different ASIC1a/2a heteromers reveals their sensitivity to the spider toxin PcTx1. Sci Rep. 2016 Jun 9;6:27647. doi: 10.1038/srep27647. DOI:10.1038/srep27647
[4] Chao Yang, et al. Overexpression of acid-sensing ion channel 1a (ASIC1a) promotes breast cancer cell proliferation, migration and invasion. Transl Cancer Res. 2020 Dec;9(12):7519-7530. DOI:10.21037/tcr-20-2115
[5] Feng Cai, et al. Acid-sensing ion channel 1a regulates the survival of nucleus pulposus cells in the acidic environment of degenerated intervertebral discs. Iran J Basic Med Sci. 2016 Aug;19(8):812-820. PMID:27746861
[6] P Escoubas, et al. Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels. J Biol Chem. 2000 Aug 18;275(33):25116-21. DOI:10.1074/jbc.M003643200

Supplier

Send Inquriy