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N,N-Bis[2-(1,1-dimethylethoxy)-2-oxoethyl]-L-glutamic Acid 1-(1,1-Dimethylethyl) 5-(Phenylmethyl) Ester is an protected intermediate in the synthesis of chelating agent for fluorescence labeling of histidine-tagged proteins to lipid membranes, which is a powerful mean for studying and monitoring protein interaction in cells.