Uses
08:0 PI(4,5)P2 is suitable for use to identify its binding site in transient?receptor potential vanilloid 4 (TRPV4?N) terminus. It has been used in in vitro binding assay to determine the binding of G-protein coupled receptor kinases (GRKs) and β2-adrenergic receptor (β2AR).
General Description
Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT. The dioctanoyl derivative of PI(4,5)P2 is more water soluble than its long chain counterparts.
