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(2S,3S)-3-Hydroxy-2-methylbutanoic Acid is an analyte in the study of patients with β-ketothiolase deficiency. It is also used in the synthesis of the pentasaccharide hapten from the glycopeptidolipid antigen of a Mycobacterium avium, an infectious bacterium.

ChEBI: (2S,3S)-3-hydroxy-2-methylbutanoic acid is a 2-methyl-3-hydroxybutyric acid that has (2S,3S)-configuration. It is a conjugate acid of a (2S,3S)-3-hydroxy-2-methylbutanoate.