83907-40-8

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SPQ offers a simple and yet powerful method to examine and measure membrane chloride transport mechanisms.

SPQ is a fluorescent chloride indicator. Its fluorescence is rapidly quenched by chloride in vitro. It has been used to assess voltage-sensitive chloride transport in placental microvillus vesicles. It has also been used to continuously quantify intracellular calcium efflux induced by parathyroid hormone, forskolin , and phorbol 12-myristate 13-acetate (PMA; ) in isolated human erythrocytes. In addition, SPQ has been used to determine the type of ion conductance induced by the cystic fibrosis transmembrane conductance regulator (CFTR) in HEK293 cells expressing pTrial10-CRTR2. SPQ displays excitation maxima of 320 and 350 nm and an emission maximum of 445 nm.

[1] NICHOLAS P. ILLSLEY  Alan S V. Membrane chloride transport measured using a chloride-sensitive fluorescent probe[J]. Biochemistry Biochemistry, 1987, 26 5: 1215-1219. DOI: 10.1021/bi00379a002
[2] LAURA SOLDATI . Chloride Fluxes Activated by Parathyroid Hormone in Human Erythrocytes[J]. Biochemical and biophysical research communications, 2000, 269 2: Pages 470-473. DOI: 10.1006/bbrc.2000.2320
[3] L J MACVINISH. Chloride secretion in the trachea of null cystic fibrosis mice: the effects of transfection with pTrial10-CFTR2.[J]. Journal of Physiology-London, 1997, 499 3: 677-687. DOI: 10.1113/jphysiol.1997.sp021960