82-94-0

ChEBI: An iminium salt composed of (4-{[4-(dimethylamino)phenyl][4-(trimethylazaniumyl)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)(dimethyl)ammonium and chloride ions in a 1:2 ratio. A histological dye used to demonstrate nucleic acids by the Unna-Pappenheim stain, in conjunction with Pyronin Y.

Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.

Methyl green is a metallic green microcrystal or bright green powder. It is soluble in water and appears bluish-green. As a basic dye, it readily binds to highly polymerized DNA, producing a green color. It is also known as Double Green SF. It appears as green crystals with a golden luster or as a light green powder. It is soluble in water and appears bluish-green. It is slightly soluble in ethanol but insoluble in ether or amyl alcohol.

Methyl green exists in two forms. Formula I: Molecular formula C26H33Cl2N3. Molecular weight 458.49. It appears as green crystals with a golden luster or as a light green powder. It is soluble in water, slightly soluble in ethanol, and insoluble in ether. It is typically a complex salt of zinc chloride. Formula II: Molecular formula: C27H35BrClN3. Molecular weight: 516.98. Green powder, soluble in water, exhibits a greenish-blue color. The solution is yellow at pH 0.2 and blue at pH 1.8.

Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.

chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction

green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.

Standard( Cotton )	Light Fastness	Persperation Fastness		Ironing Fastness		Soaping
		Fading	Stain	Fading	Stain	Fading	Stain
C	1					2

Crystallise the dye from hot water. [Beilstein 13 IV 2286.]