81493-98-3

Residues 2-12 are the v-Src (412-422) autophosphorylation site with an extra Arg for phosphocellulose binding. This substrate sequence, RRLIEDNEYTARG, was originally described as an EGFR substrate in A431 cell extracts (Km=0.3-0.6 mM).

[1] Ohguro H, et al. Separation of phospho- and non-phosphopeptides using reverse phase column chromatography. FEBS Lett. 1995 Jul 24;368(3):452-4. DOI:10.1016/0014-5793(95)00710-q
[2] Ernould AP, et al. Purification and characterization of the major tyrosine protein kinase from the human promyelocytic cell line, HL60. Eur J Biochem. 1993 Jun 1;214(2):503-14. DOI:10.1111/j.1432-1033.1993.tb17948.x
[3] L J Pike, B Gallis, et al. Epidermal growth factor stimulates the phosphorylation of synthetic tyrosine-containing peptides by A431 cell membranes. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1443-7. DOI:10.1073/pnas.79.5.1443