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Cholesta-3,5-diene was used as standard in HPLC in quality analysis of different types of olive oils and edible fats.

ChEBI: Cholesta-3,5-diene is a cholestanoid.

Cholesta-3,5-diene is an oxysterol, a cholesterol derivative by auto-oxidation. Oxysterols are non-genomic regulators of cholesterol homeostasis. The biological effects include protein prenylation, apoptosis, modulation of sphingolipid metabolism and platelet aggregation. Oxysterols bind to liver X receptors, modulate cholesterol efflux and decrease the uptake of cholesterol by the cells.