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3-epi-25-Hydroxyvitamin D₃ abbreviated as 3-epi-25(OH)D₃ is a metabolite of vitamin D₃ produced in the liver. 3-epi-25(OH)D₃ is used as a biomarker to determine the status of vitamin D in the body. It can be also employed as an analytical standard for the quantification of 3-epi-25(OH)D₃ molecules in human serum using the LC-MS/MS method.

The epimer metabolite of Calcifediol or 25-hydroxyvitamin D3 (C125700).

The labelled epimer metabolite of Calcifediol or 25-hydroxyvitamin D3 (C125700).

3-epi-25-Hydroxyvitamin D₃ (C₃-epimer) is a metabolite of vitamin D₃. It is an epimer of 25-hydroxyvitamin D₃ which differs only at C-3 position wherein -OH group?at C-3 carbon is in the α orientation rather than in the β orientation.