407627-60-5

BP Fluor 647 NHS ester (Alexa Fluor?? 647 NHS Ester equivalent) is an amine reactive bright green-fluorescent dye, it can be labeled to biomolecules directly such as primary antibody, secondary antibody, peptide, etc. The conjugates can be used as a probe for flow cytometry, immunocytochemical assays, including In-protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development. Alexa Fluor?? is registered trademarks of Thermo Fisher Scientific.

Appearance: Dark blue solid
ex/em: 651/672 nm
Extinction coefficient (ε): 261,000 L??mol??1??cm??1
Quantum yield (Φ): 0.33

AF647-NHS ester is an analog of Alexa Fluor 647 (AF647). AF647 is a far-red fluorescent dye with an excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). Storage: Protect from light[1][2].

[1] Fernandez A, et al. In vivo characterization of the biodistribution profile of amphipol A8-35. J Membr Biol. 2014;247(9-10):1043-1051. DOI:10.1007/s00232-014-9682-8
[2] Karlsson J K G, Laude A, Hall M J, et al. Photo‐isomerization of the Cyanine Dye Alexa‐Fluor 647 (AF‐647) in the Context of dSTORM Super‐Resolution Microscopy[J]. Chemistry–A European Journal, 2019, 25(65): 14983-14998.