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HPA-12 is a ceramide analog and inhibitor of ceramide transfer protein (CERT). It inhibits binding of a ceramide probe to the isolated steroidogenic acute regulatory protein-related lipid transfer (START) domain of CERT with an EC₅₀ value of 4 μM in a TR-FRET assay. HPA-12 (2.5 μM) inhibits conversion of the fluorescent ceramide analog C5-DMB-Cer to C5-DMB-sphingomyelin in CHO and HeLa cells. It also inhibits sphingomyelin synthesis in wild-type, but not ATP-dependent ceramide transport-deficient, CHO cells when used at a concentration of 1 μM.

HPA-12 is an intracellular ceramide transport protein inhibitor[1].

[1] Berke? D, et al. Chemistry and Biology of HPAs: A Family of Ceramide Trafficking Inhibitors. Chemistry. 2016 Dec 5;22(49):17514-17525. DOI:10.1002/chem.201602947