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Lactobacillus acidophilus is a probiotic bacteria. It first isolated by Moro (1900) from infant feces, The acidophilus (meaning acid-loving) bacterium is isolated from the intestinal tract of humans and animals and also is reported in the feces of milk-fed infants and older persons consuming high milk, lactose, or dextrin diets. Historically, L. acidophilus is the Lactobacillus species most often implicated as an intestinal probiotic capable of eliciting beneficial effects on the microbiota of the gastrointestinal tract (GIT). Lactobacillus acidophilus and Lactobacillus johnsonii are used in the manufacture of fermented milks commercially.