A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF-induced iPSC generation from MEFs cultured in either feeder cell-containing ESC media or in feeder-/serum-free iSF1 media (1 μM OAC1 treatment starts 48 h after viral transduction). OCA1 is shown to increase cellular
Oct4,
Nanog,
Sox2, and
Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not affect
Oct4 promoter methylation, cellular Wnt signaling, or
p53 and
p21 mRNA levels. Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat. No.
382190), Tranylcypromine (Cat. No.
616431), VPA (Cat. No.
676380), and Vitamin C (Cat. No.
1813), in comparison, do not exhibit
Oct4 and
Nanog promoters enhancing activity, while BIO (Cat. No.
361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing
Oct4 and
Nanog transcription.
Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
