2705841-52-5

8RK64 is a covalent UCHL1 inhibitor (IC50: 0.32 μM)[1]. 8RK64 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

8RK64 is a cell penetrantpotent and selective covalent inhibitor of UCHL1 (ubiquitin carboxy-terminal hydrolase L1) th at binds to the active-site cysteine residue of UCHL1. 8RK64 potently inhibits UCHL1 activity in cells.

[1] Kooij R, et al. Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos. J Am Chem Soc. 2020 Sep 30;142(39):16825-16841. DOI:10.1021/jacs.0c07726