Description
Sphingosine-
13C
2-d
2 (d18:1) is intended for use as an analytical standard for the quantification of sphingosine (d18:1) by GC- or LC-MS. Sphingosine (d18:1) is formed primarily from the breakdown of ceramide.
1 Sphingosine (d18:1) inhibits protein kinase C and phosphatidic acid phosphohydrolase, whereas it activates phospholipase D and diacylglycerol (DAG) kinase.
1 Phosphorylation of sphingosine (d18:1) by sphingosine kinases 1 and 2 (SPHK1, SPHK2) produces sphingosine-1-phosphate, a potent bioactive lipid that exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis.
2,3,4
Uses
D-erythro-Sphingosine-13C2,d2 is a deuterated labeled D-erythro-Sphingosine[1]. D-erythro-Sphingosine (Erythrosphingosine) is a very potent activator of p32-kinase with an EC50 of 8 μM, and inhibits protein kinase C (PKC). D-erythro-Sphingosine (Erythrosphingosine) is also a PP2A activator[2][3][4][5].
References
1. Hannun, Y.A., Luberto, C., and Argraves, K.M. Enzymes of sphingolipid metabolism: From modular to integrative signaling Biochemistry 40(16),4893-4903(2001).
2. Takuwa, Y., Takuwa, N., and Sugimoto, N. The Edg family G protein-coupled receptors for lysophospholipids: Their signaling properties and biological activities J. Biochem. 131(6),767-771(2002).
3. Ishii, I., Fukushima, N., Ye, X., et al. Lysophospholipid receptors: Signaling and biology Annu. Rev. Biochem. 73,321-354(2004).
4. Kluk, M.J., and Hla, T. Signaling of sphingosine-1-phosphate via the S1P/EDG-family of G-protein-coupled receptors Biochim. Biophys. Acta 1582(1-3),72-80(2002).
