2649096-17-1

Bromoenol lactone-d7 (BEL-d7) contains seven deuterium atoms at the 2, 3, 4, 5, 6, 7, and 8 positions. It is intended for use as an internal standard for the quantification of BEL by GC- or LC-MS. BEL is a selective, potent, irreversible, mechanism-based inhibitor of phospholipase A2 (iPLA2) with a Ki value of 180 nM.1 It also inhibits macrophage iPLA2 in a concentration-dependent manner with an IC50 value of 60 nM and is an effective enzyme-activated irreversible inhibitor of chymotrypsin (Ki = 636 nM).2,3WARNING This product is not for human or veterinary use.

[1] S L HAZEN. Suicide inhibition of canine myocardial cytosolic calcium-independent phospholipase A2. Mechanism-based discrimination between calcium-dependent and -independent phospholipases A2.[J]. The Journal of Biological Chemistry, 1991, 266 11: 7227-7232.
[2] E J ACKERMANN  E A D  K Conde Frieboes. Inhibition of macrophage Ca(2+)-independent phospholipase A2 by bromoenol lactone and trifluoromethyl ketones.[J]. The Journal of Biological Chemistry, 1995, 270 1: 445-450. DOI: 10.1074/jbc.270.1.445
[3] J BALSINDE. Inhibition of calcium-independent phospholipase A2 prevents arachidonic acid incorporation and phospholipid remodeling in P388D1 macrophages.[J]. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92 18: 8527-8531. DOI: 10.1073/pnas.92.18.8527