ChemicalBook CAS DataBase List 25535-16-4

25535-16-4

Name	Propidium iodide
CAS	25535-16-4
EINECS(EC#)	247-081-0
Molecular Formula	C27H34I2N4
MDL Number	MFCD00011921
Molecular Weight	668.39
MOL File	25535-16-4.mol

Synonyms

3,8-DIAMINO-5-(3-DIETHYLAMINOPROPYL)-6-PHENYLPHENANTHRIDINIUM IODIDE METHIODIDE 3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE 3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE PHENANTHRIDINIUM, 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYL-, DIIODIDE PI PROPIDIUM IODIDE 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod 3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi Propidium iodide solution Propidium diiodide PROPIDIUM IODIDE 95-98% PROPIDIUM IODIDE, FOR FLUORESCENCE PROPIDIUM IODIDE 95% -Cellstain-PiSolution -Cellstain-Pi PROPIDIUM IODIDE RED POWDER 3,8-Diamino-5-[3-(diethylmethylamino)propyl]-6-phenylphenanthridium,diiodide

Chemical Properties

Melting point	220-225 °C (dec.) (lit.)
density	1.5072 (estimate)
storage temp.	2-8°C
solubility	Difficult to mix.
form	Flaky Powder
color	Dark red
Appearance	Red solid or powder
Water Solubility	Soluble in water at 1mg/ml
ex/em	535/617 nm (and DNA)
Sensitive	Light Sensitive & Hygroscopic
Usage	A phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes
ε(extinction coefficient)	5400 cm−1M−1 (water/DNA)
λmax	493 nm, 535 nm
BRN	3843838
Biological Applications	Detecting nucleic acids,cancer cells,spores,activity of hydrolase,microorganisms; apoptosis assay; measuring beta-amyloid in Alzheimer’s disease (AD); treating diseases associated with androgens,cancer
Major Application	Electroluminescent displays; photoresists
InChIKey	ISKCJFFZOPXOFC-UHFFFAOYSA-N
SMILES	C([N+]1=C2C=C(N)C=CC2=C2C=CC(N)=CC2=C1C1C=CC=CC=1)CC[N+](C)(CC)CC.[I-]
CAS DataBase Reference	25535-16-4(CAS DataBase Reference)

Safety Data

Symbol(GHS)	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201-P202-P280-P308+P313-P405-P501
Hazard Codes	Xi,Xn
Risk Statements	R36/37/38:Irritating to eyes, respiratory system and skin . less more
Safety Statements	S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice . S36:Wear suitable protective clothing . S22:Do not breathe dust . less more
WGK Germany	3
RTECS	SF7949600
F	3-8-10-23
HS Code	29339900
Storage Class	12 - Non Combustible Liquids

Hazard Information

Description

Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques. It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

Chemical Properties

Dark Red Solid

Uses

1 mg/mL in water

Uses

Antibacterial;Detergent

Uses

Propidium Iodide is a phenanthridine compound, a class of compounds with reported activity against several species of trypanosomes. Propidium iodide may be used in flow cytometry to evaluate cell viab ility when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Uses

Propidium iodide is the most common red-fluorescent nuclear stain. It can also be excited using excitation filters appropriate for green-fluorescent dyes.

Uses

Used as fluorescent stain for nucleic acids,and used in flow cytometry to evaluate cell viability

Definition

ChEBI: Propidium iodide is an organic iodide salt. It contains a propidium.

General Description

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Biochem/physiol Actions

Primary TargetMembrane impermeable DNA intercalator

storage

+4°C

Purification Methods

It crystallises as red crystals from H2O containing a little KI. It fluoresces strongly with nucleic acids. [Watkins J Chem Soc 3064 1952, Beilstein 22 III/IV 5519.] TOXIC.

References

[1] SIMON JOHNSON David C Vy Nguyen. Assessment of Cell Viability[J]. Current Protocols in Cytometry, 2013, 64 1. DOI: 10.1002/0471142956.cy0902s64

Abs/Em Maxima

537 (complex)/616 (complex) nm

Spectrum Detail

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