2407768-11-8

AzGGK is a novel site-specific probe for ubiquitylation and sumoylation, allowing the site-specific attachment of ubls to nonrefoldable, multidomain proteins and enabling inducible and ubiquitin-ligase-independent ubiquitylation of proteins in mammalian cells

AzGGK is an unnatural amino acid. AzGGK is site-specifically incorporated into proteins via genetic-code expansion. AzGGK can be used as site-specific probe for ubiquitylation and SUMOylation[1]. AzGGK is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

[1] Fottner M, et al. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase. Nat Chem Biol. 2019 Mar;15(3):276-284. DOI:10.1038/s41589-019-0227-4