240129-30-0

7-Keto-27-hydroxycholesterol is used in the study of biochemical methods for the extraction and quantitative analysis of sterols and secosteroids from human plasma using combination of HPLC-MS and GC-MS.

7-keto-27-hydroxycholesterol (7-OC) acts as an agonist for the smoothened (SMO) protein of Hedgehog (Hh) signalling pathwaywhich is vital for proper cell differentiation in embryonic tissue. It elicits strong affinity to SMO compared to 7β,(25R)26-dihydroxycholesterol (7β,26-diHC). 7-OC is reduced to 7β,(25R)26-dihydroxycholesterol (7β,26-diHC) by reactive oxygen species (ROS).