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Methyltetrazine-amido-PEG5-alkyne is a heterobifunctional crosslinker. This reagent enables simple and efficient incorporation of a tetrazine moiety onto TCO-attached antibodies, peptide or other biomolecule under mild condition. The alkynes is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry. PEG5 space increase the compound's aqueous solubility.