ALK5 inhibitor II is a cell permeable, selective inhibitor of the TGF-
β type 1 activin like kinase receptor ALK5 (IC
50s = 4, 18, and 23 nM for ALK5 autophosphorylation, TGF-
β cellular assay, and ALK5 binding in HepG2 cells, respectively).
1 This inhibitor demonstrated less potent activity (IC
50s > 16 μM) when tested against a panel of 9 related kinases, including p38 MAPK and GSK3.
1 At 25 μM, this compound has been used to induce stem cell pluripotency by replacing the reprogramming transcription factor
Sox2 via inhibition of the TGF-
β signaling pathway and induction of
Nanog transcription.
2 This product is a hydrochloride form of the compound. A non-
hydrochloride version of ALK5 inhibitor II is also available.