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Glycyl-L-glutamine Monohydrate is the hydrated from of Glycyl-L-?Glutamine (G598125); Glycyl-L-?Glutamine is used in animal feed additives.

Glycyl-glutamine (gly-gln) is a dipeptide that is used as a substitute for glutamine in cell culture. Higher cell yields are obtained when this dipeptide is used instead of glutamine, where gly-gln affects substrate utilization and metabolism. Cell growth is higher as the concentrations of this dipeptide remain high, due to lower peptidase affinity.

Gly-Gln monohydrate has been used for the preparation of glutamine solution in HTF (human tubal fluid) medium to assess the accumulation of ammonium in commercially available culture media and protein supplements for in vitro development of human pre-implantation embryos, when stored at 2–8°C and incubated at 37°C.