BODi-1 (also known as BOBO®-1) is a cyan fluorescent carbocyanine dimeric dye. BODi-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.
The bright fluorescence signal and low background make BODi-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
The dye is used to detect and count cells (megakaryocyte, erythroblasts, sperms, microorganisms, etc.), perform nucleic acid detection, characterizing DNA/lipid complexes (lipoplexes), and as a temperature sensor.
