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PEG diacrylamide is a biocompatible, hydrophilic polymer commonly used to form crosslinked polymer networks and PEG hydrogels. Crosslinked networks can be formed via radical initiator induced polymerization and photopolymerization. Due to its stable amide bond, PEG diacrylamide can be used to prepare hydrogels suitable for long-term studies and eliminate non-specific hydrogel degradation. The acrylamide functional groups can also be reacted with thiol-containing peptide crosslinkers to create enzymatically-sensitive crosslinked networks. PEG diacrylamide has numerous applications in tissue engineering, 3D printing, and drug delivery.