Enzyme inhibitor
This paramagnetic sulfhydryl-modifying reagent (FW = 129.14 g/mol; CAS
15622-62-5; lmax = 460 nm), also known systematically as 4-amino-2,5-
dihydro-2-imino-5,5-dimethyl-1H-imidazol-1-yloxy, reacts with many
sulfhydryl-containing substances, with loss of its characteristic red color
. Porphyrexide is also be used as an oxidant substrate by a number of
enzymes, including galactose oxidase. The Escherichia coli sulfite
reductase heme protein subunit reacts at nearly unit stoichiometrry with
porphyrexide to produce a ferrisiroheme p-cation radical. Target (s) :
alcohol dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase;
a-glycerophosphate dehydrogenase; lactate dehydrogenase; and
pyruvate decarboxylase.
