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Dolichol is composed of repeated units of isoprenoids and forms a long chain unsaturated molecule. It is known to be widely present in all eukaryotic cells. Dolichol is predominantly synthesised in endoplasmic reticulum and localized to the phospholipid bilayer.

Dolichol influences the process of protein transfer in the endoplasmic reticulum through protein glycosylation. It acts as a lipid carrier of sugars.