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Arachidonoyl thio-PC is a substrate for many phospholipase A₂s (PLA₂s) including sPLA₂, cPLA₂, and iPLA₂. Cleavage of the sn-2 fatty acid by PLA₂ results in generation of a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) and DTP to allow quantitation of PLA₂ activity. Isozyme-specific cPLA₂ activity can be measured by excluding or inhibiting sPLA₂ and iPLA₂ activities in the assay.

Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay.

[1] L YU  E A D. Thio-based phospholipase assay.[J]. Methods in enzymology, 1991, 197: 65-75. DOI: 10.1016/0076-6879(91)97133-j