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24(R/S)-hydroxycholesterol-d7 has been used as an internal standard in the quantification of sterols from biological samples by gas chromatography-mass spectrometry (GC–MS). It has also been used as an internal standard for the quantification of monohydroxycholesterols by MS analysis.

24(R/S)-Hydroxycholesterol-d7 is used in the study of biochemical methods for quantitative detection of steroids containing quantitative charge tags and oxidizing agents and using mass spectroscopy for detection.

24(R/S)-hydroxycholesterol-d7 is the deuterated form of 24(R/S)-hydroxycholesterol.