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DC4 crosslinker is a mass spectrometry-cleavable crosslinking reagent (18? in length) that contains two intrinsic positive charges, which allow cross-linked peptides to fragment into their component peptides by collision-induced dissociation or in-source decay.¹ Initial fragmentation events result in cleavage on either side of the positive charges so that cross-linked peptides can be identified as pairs of ions separated by defined masses.¹ Additionally, these two intact peptide fragments can be further fragmented to yield a series of b- and y-ions for peptide identification.¹

DC4 Crosslinker is a stable crosslinking reagent with improved activity and solubility. DC4 Crosslinker is labile cleavable with collision-induced dissociation (CID), that will be fragmented into four rearrange products containing mobilized protons[1].

1. Clifford-Nunn, B., Showalter, H.D., and Andrews, P.C. Quaternary diamines as mass spectrometry cleavable crosslinkers for protein interactions J. Am. Soc. Mass Spectrom. 23(2),201-212(2012).