134452-11-2

5-Amino-6-(D-ribitylamino)uracil (5-A-RU) is a derivative of uracil (Item No. 26088) and a precursor to riboflavin.1,2 It reacts non-enzymatically with dihydroxy acetone, methylglyoxal, or glyoxal to activate mucosal-associated invariant T (MAIT) cells isolated from iVα19-Tg and iVα19Vβ6-DTg mice.2 5-A-RU (100 nmol/animal, i.p.), when administered in combination with methylglyoxal, activates MAIT cells in iVα19 Cα-/--Tg mice. It is also a substrate for F0 synthase, an enzyme involved in the biosynthesis of the energy metabolism cofactor F420 in archaea, bacteria, and eukaryotes.1WARNING This product is not for human or veterinary use.

[1] LAURE DECAMPS. Biosynthesis of F0, Precursor of the F420 Cofactor, Requires a Unique Two Radical-SAM Domain Enzyme and Tyrosine as Substrate[J]. Journal of the American Chemical Society, 2012, 134 44: 18173-18176. DOI: 10.1021/ja307762b
[2] CLAIRE SOUDAIS. In Vitro and In Vivo Analysis of the Gram-Negative Bacteria-Derived Riboflavin Precursor Derivatives Activating Mouse MAIT Cells.[J]. Journal of immunology, 2015: 4641-4649. DOI: 10.4049/jimmunol.1403224