Uses
Scrambled 10Panx (FSVYWAQADR) is a random sequence variant of a specific inhibitory peptide 10Panx targeted at the half-channel of Pannexin-1 (Panx1). Scrambled 10Panx is used as a control peptide to determine whether other experimental conditions or peptides act through specific molecular mechanisms. Scrambled 10Panx can be used for research in neurobiology and cell biology[1].
Biological Activity
scrambled 10panx is the scrambled form of 10panx (wrqaafvdsy), a mimetic peptide of pannexin 1 that inhibits dye uptake by macrophages without affecting cellular membrane currents. being structurally similar to anther peptide panxe1b, 10panx is three amino acid shorter but exerting an inhibitory effect against pannexin1 currents in oocytes to a similar extent as panxe1b. 10panx is also able to block the activation of i2nd, decrease the bradykinin-induced [ca2+]i response and attenuate bradykinin-induced atp release from human fibroblasts loaded with quinacrine. however, in several studies, scrambled 10panx has been proved to be less effective than 10panx in its authentic form.roger j. thompson, michael f. jackson, michelle e. olah, ravi l. rungta, dustin j. hines, michael a. beazely, john f. macdonald, brian a. macvicar. activation of pannexin-1 hemichannels augments aberrant bursting in the hippocampus. science 2008; 322: 1555-1559.ana rita pinheiro, diogo paramos-de-carvalho, marianna certal, cristina costa, maria teresa magalhaes-cardoso, fatima ferreirinha, maria adelina costa and paulo correia-de-sa. bradykinin-induced ca2+ signaling in human subcutaneous fibroblasts involves atp release via hemichannels leading to p2y12 receptors activation. communication and signaling 2013, 11:70junjie wang, meiyun ma, silviu locovei, robert w. keane and gerhard dahl. modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. am j physiol cell physiol 293: c1112-c1119, 2007
