1247025-84-8

MitoB is a ratiometric mass spectrometry probe that can be used for assessing changes in H₂O₂ within mitochondria in vivo. MitoB contains a triphenylphosphonium cation component that drives its accumulation in mitochondria where its arylboronic moiety selectively reacts with H₂O₂ to produce a phenol product, MitoP. Quantifying the MitoP/MitoB ratio by LC-MS/MS reflects the mitochondrial matrix H₂O₂ concentration.

MitoB is used as a probe in assessing the changes in hydrogen peroxide within mitochondria in vivo.

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[1] HELENA M COCHEMé. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila[J]. Nature Protocols, 2012, 7 5: 946-958. DOI: 10.1038/nprot.2012.035
[2] HELENA M COCHEMé. Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix.[J]. Cell metabolism, 2011, 13 3: 340-350. DOI: 10.1016/j.cmet.2011.02.003