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ChEBI: Methylene blue is an organic chloride salt having 3,7-bis(dimethylamino)phenothiazin-5-ium as the counterion. A commonly used dye that also exhibits antioxidant, antimalarial, antidepressant and cardioprotective properties. It has a role as an EC 1.4.3.4 (monoamine oxidase) inhibitor, an acid-base indicator, a fluorochrome, an antidepressant, a cardioprotective agent, an EC 3.1.1.8 (cholinesterase) inhibitor, a histological dye, an EC 4.6.1.2 (guanylate cyclase) inhibitor, an antioxidant, an antimicrobial agent, a neuroprotective agent, a physical tracer and an antimalarial. It contains a 3,7-bis(dimethylamino)phenothiazin-5-ium.

N1,N1-dimethylbenzene-1,4-diamine in Sodium thiosulfate (had better have with Aluminium sulfate) exist, the Dichromate treatment into sulfur generation sulfonic acid (2 – amino – 5 – dimethylaminobenzene thiosulfate), this product then through Dichromate and N,N-dimethylaniline processing, Into indamine – thio acid, then use Dichromate and Copper sulfate monohydrate (or MnO2) cyclization and oxidation, and finally to Zinc chloride double salt out (2C116H18N3SC1,ZnC1.H2O).

green ash. Soluble in water, slightly soluble in ethanol for blue. In concentrated sulfuric acid for yellow green, dilution for blue. Dye aqueous solution to join sodium hydroxide is purple, with dark purple precipitation.

Standard	Light Fastness	Heat-resistant(℃)		water	Sodium Carbonate(5%)	Hydrochloric acid(5%)
		Melting point	Stable
ISO	Poor			Poor