1223357-57-0

Cy5 alkyne is a cynaine linker containing an alkyne group, which enables Click Chemistry to attach deeply colored and photostable Cyanine5 fluorophore to various azide-bearing molecules. Cyanine5 is a popular fluorophore that has been widely used for various applications including intact organism imaging.

Appearance: Dark blue solid
ex/em: 640/664 nm (PBS buffer)
Extinction coefficient (ε): 250000 L??mol??1??cm??1
Quantum yield (Φ): 0.2

Cyanine5 alkyne (Alkyne-Cy5) is a fluorescent dye used to label azide proteins and can be used to analyse post-translational modifications of proteins, glycosylation etc. Cyanine5 alkyne can also be used as a mitochondrial OXPHOS inhibitor to inhibit the growth of cancer stem cells (CSC)[1][2]. Cyanine5 alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

[1] Amanda R Burnham-Marusich, et al. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis. 2014 Sep;35(18):2621-5. DOI:10.1002/elps.201400241
[2] Camillo Sargiacomo, et al. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Front Oncol. 2021 Jul 30;11:678343. DOI:10.3389/fonc.2021.678343

646/662 nm

0.2

250000

0.03

0.04

633 or 635 nm

633 or 635 nm

Alexa Fluor® 647, CF™ 647 Dye, DyLight™649