1207610-07-8

Fluorescent ligand that binds to theα4β1integrin (VLA-4) with high affinity (Kdvalues are 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+respectively). Used to detect VLA-4 affinity and conformational changes.

LDV-FITC, a fluorescent peptide, is a FITC-conjugated LDV peptide (HY-P2267). LDV-FITC binds to the α4β1 integrin with high affinity (Kd: 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+ respectively). LDV-FITC can be used to detect α4β1 integrin affinity[1][2].

Store at -20°C

[1] Hyduk SJ, et al. Phospholipase C, calcium, and calmodulin are critical for alpha4beta1 integrin affinity up-regulation and monocyte arrest triggered by chemoattractants. Blood. 2007 Jan 1;109(1):176-84. DOI:10.1182/blood-2006-01-029199
[2] Chigaev A, et al. Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn(2+) or antibody activation. J Biol Chem. 2001 Dec 28;276(52):48670-8. DOI:10.1074/jbc.M103194200