Methotrexate-alpha-alanine is a prodrug for activation by enzyme-monoclonal antibody conjugates. Methotrexate-alpha-alanine shows cytotoxicity[1][2].
References
[1] Haenseler E, et al. Activation of methotrexate-alpha-alanine by carboxypeptidase A-monoclonal antibody conjugate. Biochemistry. 1992 Jan 28;31(3):891-7. DOI:10.1021/bi00118a035 [2] Huennekens FM. Development of methotrexate alpha-peptides as prodrugs for activation by enzyme-monoclonal antibody conjugates. Adv Enzyme Regul. 1997;37:77-92. DOI:10.1016/s0065-2571(96)00011-8
