A cell-permeable pyrazole that acts as an equally potent blocker of Stim1 and Orai1-coupled CRAC (Ca
2+ release-activated Ca
2+) channel-mediated SOCE (store-operated Ca
2+ entry) in RBL-2H3 cells (IC
50 = 540 nM by Fura-2; peak current density pA/pF = -1.45 and -7.50, respectively, with or without 3 μM BTP2/Pyr3 by whole cell clamp) and the transient receptor potential cation channel TRPC3-mediated ROCE (receptor-operated Ca
2+ entry) in TRPC3-expressing HEK293 cells (IC
50 = 540 nM; by Fura-2; pA/pF = -2.27 and -18.50, respectively, with or without 3 μM BTP2/Pyr3). Pyr3 is also demonstrated to alleviate pressure overload-induced cardiac hypertrophy following TAC (transverse aortic constriction) operation in mice (0.1 mg/kg/day via i.p. osmotic pump)
in vivo. A great complement to Pyr 2 (Cat. No.
203890), Pyr6, and Pyr10 in Ca
2+ signaling studies.