Description
N6-benzoyl-Cyclic AMP (6-Bnz-cAMP) is a cell permeable cAMP analog that selectively activates PKA over exchange proteins activated by cAMP (Epac1 and 2, also known as RAPGEF3 and 4). It binds the AI and AII subunits of PKA with pKi values of 4.0 and 3.8, respectively, whereas it binds Epac1 with a pKi value of 1.3. 6-Bnz-cAMP stimulates the phosphorylation of the PKA substrate CREB but does not activate the Epac target Rap1. It can act synergistically with Epac-selective activators, such as 8-pCPT-2’-O-Me-cAMP .
Uses
N6-Benzoyladenosine 3’,5’-Cyclic Monophosphate Sodium Salt is a potent, cell-permeable PKA activator.
References
[1]lo kw, kan hm, ashe km, laurencin ct. the small molecule pka-specific cyclic amp analogue as an inducer of osteoblast-like cells differentiation and mineralization. j tissue eng regen med. 2012 jan;6(1):40-8.
[2]simone kiermayer, ricardo m. biondi,etal., epac activation converts camp from a proliferative into a differentiation signal in pc12 cells. molecular biology of the cell. vol. 16, 5639–5648, december 2005.
[3]cheng x, ji z, tsalkova t, mei f. epac and pka: a tale of two intracellular camp receptors. acta biochim biophys sin (shanghai). 2008 jul;40(7):651-62.
