Rubradirin is a polypeptide synthesis inhibitor with significant antibacterial activity. It specifically targets the peptidyl site (P site) of the ribosome and interferes with the initiation of protein synthesis by preventing the binding of fMet-tRNAf (formylmethionyl transfer RNA) to the 30S ribosomal subunit and the complete 70S ribosome in an initiation factor-dependent manner. Additionally, it dissociates already formed initiation complexes, thereby exerting its antibacterial effect[1][2].
References
[1] Reusser F. Rubradirin, an inhibitor of ribosomal polypeptide biosynthesis[J]. Biochemistry, 1973, 12(6): 1136-1142. DOI:10.1021/bi00730a019 [2] Reusser F. Rubradirin, a selective inhibitor of initiation factor dependent peptide-chain initiation[J]. Biochemistry, 1973, 12(22): 4524-4528. DOI:10.1021/bi00746a034
