106562-32-7

AMCA is fluorescent protein labeling agent. It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.

7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is a blue fluorescent dye. Its desirable properties include a relatively large Stoke's shift and resistance to photobleaching. 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in im

Non-Activated, Amine-Reactive, fluorescent probe

Primary amines (needs activation)

[1] H. KHALFAN. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins[J]. Journal of Molecular Histology, 1986, 18 9: 497-499. DOI: 10.1007/bf01675617
[2] G L FERRI. Quadruple immunofluorescence: a direct visualization method.[J]. Journal of Histochemistry & Cytochemistry, 1997, 45 2: 155-158. DOI: 10.1177/002215549704500201
[3] P. M. NEDERLOF. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences[J]. Cytometry Part B: Clinical Cytometry, 1989, 10 1: 20-27. DOI: 10.1002/cyto.990100105
[4] ANDRé GOTHOT  Jean M P  Jean Claude Grosdent. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes[J]. Cytometry Part A, 1996, 24 3: 214-225. DOI: 10.1002/(sici)1097-0320(19960701)24:3<214::aid-cyto4>3.0.co;2-h

345/450 nm

19,000 cm-1M-1

Alexa Fluor® 350, AMCA, DyLight® 350