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2,7-Dichlorodihydrofluorescein (DCFH2) is a non-fluorescent probe. 2,7-Dichlorodihydrofluorescein yields the highly fluorescent 2′,7′-dichlorofluorescein (DCF) that can be used for reactive oxygen species measurement[1][2].

[1] Reiniers MJ, et al. Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. DOI:10.1021/acs.analchem.7b00043
[2] Chen X, et al. 2',7'-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy. Free Radic Res. 2010 Jun;44(6):587-604. DOI:10.3109/10715761003709802