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外観
無色澄明の液体
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溶解性
水及びエタノールに極めて溶けやすい。
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解説
thioglycol．C2H6OS(78.14)．HSCH2CH2OH．2-メルカプトエタノール, チオグリコールともいう．エチレンオキシドに硫化水素を付加させるか，エチレンクロロヒドリンと硫化水素ナトリウムとを反応させると得られる．弱いメルカプタン臭をもつ無色の液体．沸点157 ℃，55 ℃(1.7 kPa)．d420"1.1143．nD20"1.4996．水，エタノール，エーテルに可溶．写真薬，医薬品，農薬原料に用いられる．LD50 300 mg/kg(ラット，経口)．森北出版「化学辞典（第2版）
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用途
重合調整剤、写真薬、医薬品、農薬原料
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用途
タンパク質のSH基保護剤、遺伝子工学研究、核酸抽出。
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用途
抗酸化剤ま又は還元剤として、タンパク質のスルフヒドリル基(SH-)の保護を目的として酵素反応液などに広く用いられる。また、ジスルフィド結合(-SS-)の還元的切断のためにSDS-PAGEに利用される。
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用途
還元剤、試液調製、蛍光分析用。
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用途
タンパク質のSH基保護のために用いられます。ジスルフィド結合を切断する還元剤でもあり、電気泳動試料調製時にも用いられれます。また、グアニジンチオシアン酸塩と混合することで変性作用とともに、ジスルフィド結合を切断し、RNaseを失活させる働きがあるため、組織や培養細胞からRNAを抽出する際に用いられます。
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使用上の注意
不活性ガス封入
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化学的特性
2-mercaptoethanol(bME) is a clear colorless liquid. It is a popular effective reducer, but extremely pungent.
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使用
2-Mercaptoethanol is used in the preparation of nano-graphene for cellular imaging and drug delivery as well as multifunctional polymeric micelle. It acts as a reducing agent used in electrophoresis, amino acid detection, and distinguishing ssDNA/dsDNA. It is also employed as a standard buffer. It is also used in the preparation of PVC heat stabilizers and as a chain transfer agent in the manufacture of PVC. Further, it is used in some RNA isolation procedures to eliminate ribonuclease. It is utilized as a corrosion inhibitor and ore floatation agent. In biochemistry, it is useful to study the activity of the immune system. Solubilizes proteins by reducing disulfide linkages.
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主な応用
2-Mercaptoethanol is used in the synthesis of nano-graphene for cellular imaging and drug delivery. Also used in the synthesis of multifunctional polymeric micelle used in specific targeting of tumor disruption.
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定義
ChEBI: 2-Mercaptoethanol is a primary alcohol and an alkanethiol. It has a role as a geroprotector.
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一般的な説明
A water-white liquid. May be toxic by ingestion, inhalation, or skin absorption.
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空気と水の反応
No rapid reaction with air. No rapid reaction with water.
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反応プロフィール
Organosulfides, such as 2-Mercaptoethanol, are incompatible with acids, diazo and azo compounds, halocarbons, isocyanates, aldehydes, alkali metals, nitrides, hydrides, and other strong reducing agents. Reactions with these materials generate heat and in many cases hydrogen gas. Many of these compounds may liberate hydrogen sulfide upon decomposition or reaction with an acid.
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危険性
Toxic by inhalation and ingestion.
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健康ハザード
TOXIC; inhalation, ingestion or skin contact with material may cause severe injury or death. Contact with molten substance may cause severe burns to skin and eyes. Avoid any skin contact. Effects of contact or inhalation may be delayed. Fire may produce irritating, corrosive and/or toxic gases. Runoff from fire control or dilution water may be corrosive and/or toxic and cause pollution.
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火災危険
Combustible material: may burn but does not ignite readily. When heated, vapors may form explosive mixtures with air: indoors, outdoors and sewers explosion hazards. Contact with metals may evolve flammable hydrogen gas. Containers may explode when heated. Runoff may pollute waterways. Substance may be transported in a molten form.
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安全性プロファイル
Poison by ingestion,
skin contact, and intraperitoneal routes.
Moderately toxic by intravenous route. A
skin and severe eye irritant. Human
mutation data reported. A combustible
liquid when exposed to heat, flame, or
oxidizers. To fight fire, use alcohol foam,
CO2, dry chemical. When heated to
decomposition it emits highly toxic fumes of
SOx. See also MERCAPTANS.
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純化方法
Purify it by distilling in a vacuum. Distilling at atmospheric pressure causes some oxidation and should be done in an inert atmosphere. [Woodward J Chem Soc 1892 1948.] It has a foul odour, is irritating t o the eyes, nose and skin — should be handled in an efficient fume cupboard. It is miscible with H2O, EtOH, Et2O and *C6H6 and the UV has max at 235nm. The 2,4-dinitrophenyl thioether has m 101-102o (from EtOH or aqueous MeOH) [Grogen et al. J Org Chem 20 50 1955]. [Beilstein 1 IV 2428.]
