荧光钙探针 Fura 2-AM
荧光钙探针 Fura 2-AM 性质
| 熔点 | >250℃ |
|---|---|
| 沸点 | 975.9±75.0 °C(Predicted) |
| 密度 | 1.400±0.06 g/cm3(Predicted) |
| 储存条件 | −20°C |
| 溶解度 | 可溶于DMSO |
| 酸度系数(pKa) | 1.89±0.50(Predicted) |
| 形态 | 粉末 |
| 颜色 | 粘黄色 |
| 最大波长(λmax) | 370nm |
| 敏感性 | Light Sensitive |
| BRN | 8183748 |
| 生物领域应用 | Calcium indicator; monitoring membrane potential; treating ischemia |
| CAS 数据库 | 108964-32-5(CAS DataBase Reference) |
荧光钙探针 Fura 2-AM 用途与合成方法
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6. Mount the coverslip on the imaging chamber.
荧光钙探针 Fura 2-AM 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024-01-25 | HY-101897 | 荧光钙探针 Fura 2-AM | 108964-32-5 | 1mg | 5000 |
