2-[4-(2-ヒドロキシエチル)-1-ピペラジニル]エタンスルホン酸

外観

白色吸湿性結晶性粉末

定義

本品は、次の化学式で表される複素環式化合物である。

溶解性

水によく溶ける(11.91g/50ml水)。0℃において2.25mol/lで飽和する。有機溶媒にはほとんど溶けない。

化粧品の成分用途

緩衝剤

説明

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

化学的特性

White crystalline powder

使用

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.

定義

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.

一般的な説明

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

生物活性

HEPES is a multi purpose HEPES buffer used in cell culture and other biological research. Working pH range in aqueous solution: 6.8 - 8.2. Does not form complexes with metal ions. Used in cell culture media.

純化方法

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]