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解説
生殖腺刺激ホルモンの一。脳下垂体前葉から分泌されるホルモンで、卵巣の卵胞を成熟させ、エストロゲンの分泌を促進させる。FSH（follicle-stimulating hormone）。
小学館 デジタル大辞泉について 情報 | 凡例
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説明
Gonal-F was launched in Austria, Germany, Denmark, Ireland, Italy,
Norway, Sweden, Switzerland, France and the UK for the treatment of infertility. The
recombinant follicule stimulating hormone (recFSH) was produced by introducing the
genes for the α- and β-FSH subunits into the genome of Chinese hamster ovary cells.
The isolated recFSH was a glycosylated bioactive dimer that appeared to be identical
to urinary human FSH. Biochemically, the recFSH was equal to or slightly better to
the uroFSH. The ultra-high purity of recFSH makes it less prone to side effects from
contaminants in uroFSH and can be injectable.
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化学的特性
Solid; soluble in water.
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使用
Follicle stimulating hormone (FSH) from human pituitary has been used for-
- in vitro luteinization of Macaque granulosa cells
- luteal cell proliferation assay
- culturing of preantral follicle cells obtained from mice
- in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
- culture of RAW264.7 cell line
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定義
A gonadotropic glycoprotein secreted by the anterior
pituitary gland. It regulates the metabolic activity of
the granulosa of the mammalian ovary; stimulates
maturation of ovarian follicles, and production of
estrogens. Spermatogenesis is partially under
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生物学の機能
Follitropins are hormonal products that consist entirely of FSH and are used to stimulate ovarian
follicle growth in women who do not have primary ovarian failure. In the absence of an adequate
endogenous LH surge, however, hCG must be given following the use of follitropins to stimulate
ovulation.
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一般的な説明
The α chain of FSH?has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.
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臨床応用
The gonadotropin follicle-stimulating hormones (FSH), follitropin alfa (Gonal-F) and follitropin beta (Follistim), are produced in the anterior lobe of the pituitary gland. FSH can function in two ways. On the one hand, it causes increased spermatogenesis in men. On the other hand, in concert with estrogen and luteinizing hormone (LH), it stimulates follicular growth and development in women. Consequently, FSH may be useful in the treatment of infertility. FSH is a member of a superfamily of proteins, all structurally related, which includes LH, chorionic gonadotropin, and thyroid-stimulating hormone (TSH).
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純化方法
FSH is purified by Sephadex G100 gel filtration followed by carboxymethyl-cellulose with NH4OAc pH 5.5. The latter separates luteinising hormone from FSH. Its solubility in H2O is 0.5%. It has an isoelectric point of 4.5. A solution of 1mg in saline (100mL) can be kept at 60o for 0.5hour. Activity is retained in a solution at pH 7-8 for 0.5hour at 75o. The activity of a 50% aqueous EtOH solution is destroyed at 60o in 15minutes. [Bloomfield et al. Biochim Biophys Acta 533 371 1978, Hartree Biochem J 100 754 1966, Pierce & Parsons Ann Rev Biochem 50 465 1981.]
