六氢姜黄素

生物活性靶点体外研究体内研究六氢姜黄素试剂级价格

六氢姜黄素

CAS号:36062-05-2
英文名:HEXAHYDROCURCUMIN
中文名:六氢姜黄素
CBNumber:CB41412369
分子式:C21H26O6
分子量:374.43
MOL File:36062-05-2.mol

六氢姜黄素化学性质

熔点 :80-82℃
沸点 :622.6±55.0 °C(Predicted)
密度 :1.226
闪点 :218℃
储存条件 :2-8°C
溶解度 :Chloroform (Sparingly), Ethyl Acetate (Slightly), DCM, DMSO
酸度系数(pKa) :10.02±0.20(Predicted)
颜色 :Pale Yellow
BRN :2486876

安全信息

WGK Germany :3

六氢姜黄素性质、用途与生产工艺

生物活性 Hexahydrocurcumin 是姜黄素的主要代谢产物之一，是一种选择性的口服活性 COX-2 抑制剂，对 COX-1 无活性。Hexahydrocurcumin 具有抗氧化，抗癌和抗炎的作用。
靶点

COX-2

体外研究

Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) treatment significantly decreased the viability of HT-29 colon cancer cells in a time- and concentration-dependent. The respective IC ₅₀ values for 24 and 48 h of Hexahydrocurcumin exposureare 77.05 and 56.95, respectively.
Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 µM) markedly reduces the COX-2 expression. The level of COX-1 is not altered.
Hexahydrocurcumin (0-25 μM; 24-48 hours; HT-29 cells) combined with 5-fluorouracil (5-FU; 5 µM) markedly reduces the COX-2 protein. The level of COX-1 protein is not altered.
Hexahydrocurcumin (7-14 μM; 24 hours) attenuates lipopolysaccharide (LPS)-elicited increase of prostaglandin E ₂ (PGE ₂ ) in murine macrophages (RAW 264.7) in a concentration-dependent manner.

Cell Viability Assay

Cell Line:	HT-29 cells
Concentration:	0 µM, 5 µM, 10 µM, 25 µM
Incubation Time:	24 hours or 48 hours
Result:	Significantly decreased the viability of HT-29 colon cancer cells.

RT-PCR

Cell Line:	HT-29 cells
Concentration:	25 µM
Incubation Time:	24 hours
Result:	Combined with 5-fluorouracil (5-FU; 5 µM) markedly reduced the COX-2 expression.

Western Blot Analysis

Cell Line:	HT-29 cells
Concentration:	25 µM
Incubation Time:	24 hours
Result:	Combined with 5-fluorouracil (5-FU; 5 µM) markedly reduced the COX-2 protein.

体内研究

Hexahydrocurcumin (50 mg/kg; oral administration; daily; for 16 weeks; male Wistar rats) treatment significantly reduces the numbers of aberrant crypt foci (ACF) in colon cancer rats. Hexahydrocurcumin also markedly decreases COX-2 protein expression. The levels of COX-1 protein is not different from normal rats.

Animal Model:	Male Wistar rats (100-120 g) injected with dimethylhydrazine (DMH)
Dosage:	50 mg/kg
Administration:	Oral administration; daily; for 16 weeks
Result:	Significantly reduced the numbers of ACF in colon cancer rats. Also markedly decreased COX-2 protein expression.