1,4-二亞硝哌【口+井】

生物活性体外研究体内研究 1,4-二亞硝哌【口+井】试剂级价格

1,4-二亞硝哌【口+井】

CAS号:140-79-4
英文名:N,N'-DINITROSOPIPERAZINE
中文名:1,4-二亞硝哌【口+井】
CBNumber:CB0420996
分子式:C4H8N4O2
分子量:144.13
MOL File:140-79-4.mol

1,4-二亞硝哌【口+井】化学性质

熔点 :208-210°C
沸点 :262.72°C (rough estimate)
密度 :1.4165 (rough estimate)
折射率 :1.8500 (estimate)
储存条件 :-20°C Freezer, Under Inert Atmosphere
溶解度 :Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly)
形态 :Solid
酸度系数(pKa) :-4.82±0.70(Predicted)
颜色 :White to Pale Yellow
水溶解性 :5.765g/L(24 ºC)
稳定性 :Hygroscopic
CAS 数据库 :140-79-4
EPA化学物质信息 :Piperazine, 1,4-dinitroso- (140-79-4)

安全信息

毒害物质数据 :140-79-4(Hazardous Substances Data)

1,4-二亞硝哌【口+井】性质、用途与生产工艺

生物活性 N,N'-Dinitrosopiperazine (1,4-Dinitrosopiperazine; DNP) 是一种致癌物 (carcinogen)，特异性针对鼻咽癌，可促进鼻咽癌的转移。N,N'-Dinitrosopiperazine 通过蛋白磷酸化调节多种信号通路，包括 LYRIC，在丝氨酸 568 位。

体外研究

N,N'-Dinitrosopiperazine (0.5-100 μM; 48 hours) has no inhibitory effects on the labeled 6-10B cells, and LDH activity is not significantly altered by DNP treatment in the 0.5-8 μM concentration range. However, it is cytotoxic from the concentration 10 μM.N,N'-Dinitrosopiperazine (2-8 μM; 24 hours) induces 6-10B cell invasion and motility in a dose-dependent manner. At 6 μM, when compares to the control group, DNP increases cell invasion at 421.7% and cell motility is increased by 328.2%.N,N'-Dinitrosopiperazine (6 μM; 24 hours) increases the expression of phospho-LYRIC s568 and LYRIC expression in CNE1 cells.

Cell Viability Assay

Cell Line:	The labeled 6-10B cells
Concentration:	0.5, 1, 2, 4, 6, 8, 10, 20, 40, 80, or 100 μM
Incubation Time:	48 hours
Result:	Had no inhibitory effects at the concentration 0-8 μM.

Western Blot Analysis

Cell Line:	The NPC cell line CNE1
Concentration:	6 μM
Incubation Time:	24 hours
Result:	Increased phospho-LYRIC s568 and LYRIC expression.

体内研究

N,N'-Dinitrosopiperazine (injected into the tail veins; 40 mg/kg; 30 days) inhibits cell motility and invasion, and facilitates NPC metastasis in vivo. From a IHC result, Phospho-LYRIC expression is higher in the metastatic tumors of DNP-treated mice than in those of the untreated control mice.

Animal Model:	BABL/c nude mice injected with labeled 6-10B cell suspensions (1 × 10 ⁴ cells) with or without DNP(40 mg/kg)
Dosage:	40 mg/kg
Administration:	Injected into the tail veins; 30 days
Result:	Induced LYRIC phosphorylation at serine 568 associated with NPC metastasis in vivo.