APS-5

Описание

Lumigen APS-5 is a proprietary acridan based chemiluminescent substrate for ELISA detection of alkaline phosphatase (AP) conjugated molecules. The unique acridan chemistry of Lumigen APS-5 provides temperature insensitive light output and more rapid peak intensity as compared to the dioxetane based AP substrates, Lumi-Phos 530 and Lumi-Phos Plus.

Использование

Aps-5 is a chemiluminescent substrate based on 9, 10-dihydroacridine, which is mainly used for ELISA detection of compounds with alkaline phosphatase AP conjugation.The 9, 10-dihydroacridine structure in the aps-5 structure can continuously, stably and efficiently give off light under the action of AP, and aps-5 is very suitable for the determination of phosphatase activity solution and phosphatase-linked immunoassay.

Механизм действия

Lumigen APS-5 utilizes a unique technology for the chemiluminescent detection of alkaline phosphatase conjugates which combine excellent sensitivity with ease of use. Reaction of the acridan substrate with an AP label rapidly produces sustained high-intensity chemiluminescence. Lumigen APS-5 is ideally suited for solution assays of phosphatase activity and for phosphatase-linked immunoassays.